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KMID : 0613820020120060715
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Journal of Life Science
2002 Volume.12 No. 6 p.715 ~ p.720
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Mapping of UV-B sensitive gene in Arabidopsis by CAPS markers
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Park Hong-Duck
Kim Jong-Bong
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Abstract
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A mutant Arabidopsis thaliana which is very sensitive to Ultraviolet-B(UV-B) radiation has been isolated by ethylmethane sulfonate(EMS) mutagenesis. Genetic cross proved the UV-sensitive gene(uvs) to segregate as a single Mendelian locus. For mapping of uvs, we crossed Arabidopsis thaliana Lansberg with uvs plant(Columbia), and made F2 plants by F1 selfcross. We designed 10 kinds of CAPS marker primers. Each primers amplifies a single mapped DNA sequence from uvs and Lansberg erecta ecotyres. Also identified was at least one restriction endonuclase for each of these PCR product that generates ecotype-specific digestion pattern. We got crossing over value of UB-sensitivity and each CAPS marker which located on different chromosome arm. The value of crossing over showed that uvs was linked to LFY3 which was on chromosome 5.
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KEYWORD
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Arabidopsis, ultraviolet, CAPS marker, Mapping
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